Iodine Test
USING HANNA CHECKER HI-707 TO TEST FOR IODINE
THE PROCEDURE
1) Hanna HI-707 Nitrite LR Checker
2) Red Sea Iodine Pro Reagents A & B
3) Two Hanna Checker Vials
4) Timer
5) Accurate Pipette(s) or Syringe cable of measuring .5 mL and .8 mL
6) RODI Water for rinsing vials & pipettes
7) Accurate Thermometer
8) Sample Water Container (I use a beaker or plastic bottle)
PROCEDURE…STEP BY STEP (See Included Video)
1) Fill vial with water to be tested. This will be the Blank. Place it into the Checker
2) Set Timer to 1 Minute 45 seconds
3) Rinse second vial with water to be tested
4) Fill second vial with water to be tested.
5) Add .5 mL of reagent “A” to second vial containing the test water.
6) Add .8 mL of reagent “B’ to the second test vial — IMMEDIATELY START TIMER!!
7) Put the cap on the second vial and invert the vial several times
8) Press the button on the Hanna Checker…When “C1” appears press again….”C2” will now be on the display.
9) Remove the blank vial from the checker and put the second vial into the checker.
10) The Timer should be still counting down from 1:45…When the timer reaches “0” IMMEDIATELY PRESS THE BUTTON ON THE CHECKER TO GET THE READING
11) Record the value appearing on the meter…This is the value you put onto the formula below.
12) MEASURE AND RECORD THE TEMPERATURE OF THE SAMPLE BEING TESTED
NOTE ON TEMPERATURE: Temperature below 73⁰F at lower iodine (<.02ppm) concentration levels will cause the HI-707 to exceed its measurement limits of 600. It is recommended to keep the sample temperature above 73⁰F. See the section on the “Regression Equations” for details in calculating the PPM iodine.
Multiply the meter reading by -.0003292492 then add 0.1973653 This will = PPM Iodine
Example: (No Temperature Correction)
Meter Reading 422
1) 422 x -0.0003292492 = -0.138943162
2) -0.138943162 + 0.1973653 = 0.058 PPM Iodine
NOTE ON TEMPERATURE: Temperature below 73⁰F at lower iodine (<.02ppm) concentration levels will cause the HI-707 to exceed its measurement limits of 600. It is recommended to keep the sample temperature above 73⁰F. See the section on the “Regression Equations” for details in calculating the PPM iodine.
1) Hanna HI-707 Nitrite LR Checker
2) Red Sea Iodine Pro Reagents A & B
3) Two Hanna Checker Vials
4) Timer
5) Accurate Pipette(s) or Syringe cable of measuring .5 mL and .8 mL
6) RODI Water for rinsing vials & pipettes
7) Accurate Thermometer
8) Sample Water Container (I use a beaker or plastic bottle)
PROCEDURE…STEP BY STEP (See Included Video)
1) Fill vial with water to be tested. This will be the Blank. Place it into the Checker
2) Set Timer to 1 Minute 45 seconds
3) Rinse second vial with water to be tested
4) Fill second vial with water to be tested.
5) Add .5 mL of reagent “A” to second vial containing the test water.
6) Add .8 mL of reagent “B’ to the second test vial — IMMEDIATELY START TIMER!!
7) Put the cap on the second vial and invert the vial several times
8) Press the button on the Hanna Checker…When “C1” appears press again….”C2” will now be on the display.
9) Remove the blank vial from the checker and put the second vial into the checker.
10) The Timer should be still counting down from 1:45…When the timer reaches “0” IMMEDIATELY PRESS THE BUTTON ON THE CHECKER TO GET THE READING
11) Record the value appearing on the meter…This is the value you put onto the formula below.
12) MEASURE AND RECORD THE TEMPERATURE OF THE SAMPLE BEING TESTED
NOTE ON TEMPERATURE: Temperature below 73⁰F at lower iodine (<.02ppm) concentration levels will cause the HI-707 to exceed its measurement limits of 600. It is recommended to keep the sample temperature above 73⁰F. See the section on the “Regression Equations” for details in calculating the PPM iodine.
Multiply the meter reading by -.0003292492 then add 0.1973653 This will = PPM Iodine
Example: (No Temperature Correction)
Meter Reading 422
1) 422 x -0.0003292492 = -0.138943162
2) -0.138943162 + 0.1973653 = 0.058 PPM Iodine
NOTE ON TEMPERATURE: Temperature below 73⁰F at lower iodine (<.02ppm) concentration levels will cause the HI-707 to exceed its measurement limits of 600. It is recommended to keep the sample temperature above 73⁰F. See the section on the “Regression Equations” for details in calculating the PPM iodine.
NOTES ON THE PROCEDURE: The time is very critical to achieving accurate and precise results so paying attention to start and stop times is important. In addition the accurate measurement of the reagents is important to getting good results. I generally run two tests back to back and take an average of the values. If the two results are greater than 5% from each other I run a third test and take the average of the 3 as my value…This does not happen very often. (See Table 3) Total testing time is approximately 6-8 minutes….One note on the video. The video shows Step 7 and 8 in different order than the procedure calls for…The procedure is the correct way.
